Katharine Hepburn
Mary Stuart
Acting
Florence Eldridge
Elizabeth Tudor
Acting
Fredric March
Bothwell
Acting
Douglas Walton
Darnley
Acting
John Carradine
Rizzio
Acting
Robert Barrat
Morton
Acting
Gavin Muir
Leicester
Acting
Ian Keith
Moray
Acting
Moroni Olsen
John Knox
Acting
William Stack
Ruthven
Acting
Ralph Forbes
Randolph
Acting
Alan Mowbray
Throckmorton
Acting
Frieda Inescort
Mary Beaton
Acting
Donald Crisp
Huntly
Acting
David Torrence
Lindsay
Acting
Molly Lamont
Mary Livingstone
Acting
Anita Colby
Mary Fleming
Acting
Jean Fenwick
Mary Seton
Acting
Lionel Pape
Burghley
Acting
Alec Craig
Donal
Acting
Mary Gordon
Nurse
Acting
Monte Blue
Messenger
Acting
Robert Warwick
Sir Francis Knollys
Acting
Halliwell Hobbes
Man (uncredited)
Acting
Nigel De Brulier
Judge
Acting