Josh Keaton
Peter Parker/Spider-Man (voice)
Acting
Lacey Chabert
Gwen Stacy (voice)
Acting
James Arnold Taylor
Harry Osborn (voice)
Acting
Alan Rachins
Norman Osborn (voice)
Acting
Deborah Strang
Aunt May Parker (voice)
Acting
Ben Diskin
Eddie Brock (voice)
Acting
Dee Bradley Baker
Curt Connors/Lizard (voice)
Acting
Robert Englund
Adrian Toomes/Vulture (voice)
Acting
Crispin Freeman
Max Dillon/Electro (voice)
Acting
Jeff Bennett
Montana/Shocker (voice)
Acting
Daran Norris
J. Jonah Jameson (voice)
Acting
Joshua LeBar
Flash Thompson (voice)
Acting
Peter MacNicol
Otto Octavius/Doctor Octopus (voice)
Acting
Alanna Ubach
Liz Allen (voice)
Acting
Grey DeLisle
Sally Avril (voice), Betty Brant (voice)
Acting
Clancy Brown
Alex O'Hirn/Rhino (voice)
Acting