Bela Lugosi
Scientist
Acting
Edward D. Wood Jr.
Glen / Glenda (as Daniel Davis)
Writing
Lyle Talbot
Inspector Warren
Acting
Timothy Farrell
Dr. Alton / Narrator
Acting
Dolores Fuller
Barbara
Acting
'Tommy' Haynes
Alan / Anne
Acting
Charlie Crafts
Johnny (as Charles Crafts)
Acting
Conrad Brooks
Banker / Reporter / Pickup Artist / Bearded Drag (as Connie Brooks)
Acting
Henry Bederski
Man with Hat and Receding Hairline (uncredited)
Acting
Carol Daugherty
Woman in Nightmare (uncredited)
Acting
'Captain' DeZita
The Devil / Glen's Father (uncredited)
Acting
Helen Miles
(uncredited)
Acting
Shirley Speril
Miss Stevens (uncredited)
Acting
Amzie Strickland
Minor Role (uncredited)
Acting
Harry Thomas
Man in Nightmare (uncredited)
Costume & Make-Up
William C. Thompson
Judge (uncredited)
Camera
'Mr. Walter' Hadjwiecyz
Patrick / Patricia (uncredited)
Acting
George Weiss
Man at Transvestite's Suicide (uncredited)
Production
Evelyn Wood
Sheila, Glen's Sister (uncredited)
Acting